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Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice

Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice

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06:23 min

June 05, 2021

DOI:

06:23 min
June 05, 2021

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Summary

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The shrinkage of dental cement during curing displaces the baseplate. This protocol minimizes the problem by creating an initial foundation of the dental cement that leaves space to cement the baseplate. Weeks later, the baseplate can be cemented in position on this scaffold using little new cement, therebyreducing shrinkage.

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