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Kontaktfri co-kulturmodel til undersøgelse af medfødt immuncelleaktivering under luftvejsvirusinfektion
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1Department of Otolaryngology, Yong Loo Lin School of Medicine,National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Haartman Institute, Medicum,University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital,National University Health System, 5Agency for Science, Technology and Research (A*STAR),Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore
Capítulos
- 00:04Introduction
- 00:50hNEC Viral Infection and hNEC + PBMCs Co-Culture
- 03:30Harvesting of PBMCs 48/72 h Post-Viral Infection
- 05:31Results: Activation of T Cells Following Influenza Infection
- 06:56Conclusion
Denne protokol beskriver en undersøgelse af de tidlige interaktioner mellem viralt inficerede nasale epitelceller og medfødt celleaktivering. Individuelle undergrupper af immunceller kan skelnes ud fra deres aktivering som reaktion på virusinfektioner. De kan derefter undersøges yderligere for at bestemme deres virkninger på tidlige antivirale reaktioner.
