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CRISPR/Cas9基因组编辑的半球藻（ Hübner）的胚胎显微注射和敲除突变体鉴定

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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

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CRISPR/Cas9基因组编辑的半球藻（ Hübner）的胚胎显微注射和敲除突变体鉴定

4,103 Views

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06:37 min

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July 01, 2021

DOI:

10.3791/62068-v

DOI:

10.3791/62068-v

•

06:37 min

July 01, 2021

•

1 Views

Dong Ai², Bing Wang, Zhennan Fan, Yuyao Fu, Caihong Yu, Guirong Wang³

¹School of Chemical and Environmental Engineering,China University of Mining and Technology, ²State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, ³Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences

Tags

CRISPR/Cas9 Genome Editing Helicoverpa Armigera Cotton Bollworm Embryo Microinjection Knockout Mutant Identification SgRNA Target Selection PAM Sequence Embryo Preparation Egg Collection Microinjection Cas9 Protein SgRNA

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