Journal
/
Quimica
/
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
JoVE Revista
Quimica
This content is Free Access.
JoVE Revista Quimica
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

DOI:
10.3791/61760-v

•

07:54 min

•

October 27, 2020

•

, , , , , ,
1School of Geography Earth and Environmental Sciences,University of Birmingham, 2Biomedical Microscale Analytics,Leiden University, 3Institute of Medical Biochemistry,Medical University of Innsbruck, 4AB Sciex UK Ltd

Capítulos

  • 00:04Introduction
  • 00:30Biomolecular Corona Formation and Protein Corona Isolation
  • 02:52Metabolite Corona Isolation
  • 03:50Preparation of CESI Capillaries and CE-MS for Protein Corona Analysis
  • 05:11Preparation of CESI Capillaries and CESI-MS for Metabolite Corona Analysis
  • 06:27Results: Mass Spectral Analysis of the Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
  • 07:26Conclusion

Summary

Traducción Automática

Traducción Automática

Here we present a protocol to characterize the complete biomolecular corona, proteins, and metabolites, acquired by nanomaterials from biofluids using a capillary electrophoresis – mass spectrometry approach.

Tags

Keywords: Capillary ElectrophoresisMass SpectrometryCESI-MSProtein CoronaMetabolite CoronaNanomaterialsHuman PlasmaProtein CharacterizationMetabolite CharacterizationTrypsin DigestionC18 DesaltingSample Preparation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/61760?article_type=v

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
check_url/es/61760?article_type=v

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
check_url/es/61760?article_type=v

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
check_url/es/61760?article_type=v

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
check_url/es/61760?article_type=v

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
check_url/es/61760?article_type=v

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
check_url/es/61760?article_type=v

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
check_url/es/61760?article_type=v

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
check_url/es/61760?article_type=v

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
check_url/es/61760?article_type=v

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Read Article