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Inmunología y contagio
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Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

DOI:
10.3791/61679-v

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06:48 min

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December 04, 2020

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, , , ,
1Department of Physiology, Development and Neuroscience,University of Cambridge

Capítulos

  • 00:04Introduction
  • 01:04Larval Ventral Fin Wounding and Live Imaging Preparation
  • 02:22Live Confocal Imaging
  • 03:03Zebrafish Neutrophil Receptor Internalization Quantification
  • 04:36Results: Representative Differential CXCR1 and CXCR2 Wound Response Dynamics
  • 06:12Conclusion

Summary

Traducción Automática

Traducción Automática

Here we describe protocols to perform live imaging and quantitative analysis of chemoattractant receptor dynamics in zebrafish neutrophils

Tags

ZebrafishNeutrophilsWound ResponseChemokine ReceptorsLive ImagingIn VivoInflammationImmune ResponseTransgenicFin WoundingConfocal MicroscopyTime-lapse ImagingReceptor Internalization

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