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Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
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1Program of Biomedical Science, Graduate School of Integrated Sciences for Life,Hiroshima University, 2Institute for Quantum Life Science,National Institute for Quantum and Radiological Science and Technology, 3National Institute for Radiological Sciences,National Institute for Quantum and Radiological Science and Technology, 4JST, PRESTO
Capítulos
- 00:04Introduction
- 00:32Cultivation of Worms and Hardware Setup for Calcium Imaging
- 01:46Software Setup for Calcium Imaging
- 03:22Preparation of Worms and Calcium Imaging
- 04:37Data Analysis
- 05:23Results: Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
- 06:19Conclusion
Reported here is a system for calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibration. This system allows researchers to evoke nonlocalized vibrations with well-controlled properties at nano-scale displacement and to quantify calcium currents during responses of C. elegans to the vibrations.
