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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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JoVE Revista Bioquímica
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

DOI:
10.3791/61603-v

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09:09 min

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March 05, 2021

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, ,
1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education),Tsinghua University

Capítulos

  • 00:05Introduction
  • 00:48Microscope Slide Preparation
  • 02:06Single Particle Darkfield Microscopy (DFM) Tracking
  • 02:53Data Acquisition
  • 04:21Red/Green Values
  • 04:59Data Analysis
  • 05:30Mean Square Displacement (MSD), Multi-Particle, and Time Analyses
  • 06:33Results: Representative Single Nanoparticle DFM of AuNR Diffusion Dynamics
  • 08:39Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we show the use of traditional dark-field microscopy to monitor the dynamics of gold nanorods (AuNRs) on cell membrane. The location and orientation of single AuNRs are detected using ImageJ and MATLAB, and the diffusive states of AuNRs are characterized by single particle tracking analysis.

Tags

Gold NanorodsSingle NanoparticleDarkfield MicroscopyCell MembraneDiffusion DynamicsTranslational DynamicsRotational DynamicsSingle Particle TrackingCTAB CoatingCell CultureImage ProcessingImageJ

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