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三次元運動神経オルガノイド発生

JoVE Revista
Neurociencias

This content is Free Access.

JoVE Revista Neurociencias

Three-Dimensional Motor Nerve Organoid Generation

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三次元運動神経オルガノイド発生

8,081 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:

10.3791/61544-v

•

09:57 min

September 24, 2020

•

61 Views

Tatsuya Osaki^1,2, Siu Yu A. Chow^1,2, Yui Nakanishi^1,2, Joel Hernández^1,3, Jiro Kawada⁴, Teruo Fujii¹, Yoshiho Ikeuchi^1,2

¹Institute of Industrial Science,The University of Tokyo, ²Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, ³Faculty of Science and Engineering,Tecnologico de Monterrey, ⁴Jiksak Bioengineering, Inc.

Summary

このプロトコルは、組織培養チップ内のスフェロイドから伸びる強い軸索束の自発的な組み立てを通じて、ヒトiPS細胞由来運動神経オルガノイドを作製するための包括的な手順を提供する。

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