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Journal / Immunology and Infection / Analyse af Human Natural Killer Cell Metabolisme
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse af Human Natural Killer Cell Metabolisme
 
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Analyse af Human Natural Killer Cell Metabolisme

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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I dette papir beskriver vi en metode til at måle glykolyse og mitokondriel respiration i primære humane Natural Killer (NK) celler isoleret fra perifert blod, i hvile eller efter IL15-induceret aktivering. Den beskrevne protokol kan let udvides til primære humane NK-celler, der aktiveres af andre cytokiner eller opløselige stimuli.

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Immunologi og infektion Natural Killer celler stofskifte ekstracellulære flux glykolyse mitokondrier respiration elektron transport kæde flow cytometri
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