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X射线晶体学研究热木体马里蒂玛M42氨基肽TmPep1050的寡聚态过渡
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X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
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X射线晶体学研究热木体马里蒂玛M42氨基肽TmPep1050的寡聚态过渡

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11:27 min

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May 13, 2020

DOI:

10.3791/61288-v

DOI:
10.3791/61288-v

•

11:27 min
May 13, 2020

•

2 Views
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1Laboratory of Microbiology, Department of Molecular Biology,Université Libre de Bruxelles, 2Labiris Institut de Recherche

Summary

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该协议已经开发，以研究TmPep1050的二丁二代过渡，一种M42氨基肽，在结构层面。从蛋白质纯化到 X 射线数据处理，这是一条简单的管道。通过一个案例研究，TmPep1050H60A H307A 变种强调了晶体生成、数据集索引和分子 替代。

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Cite this Article

Dutoit, R., Brandt, N., Van Elder, D., Droogmans, L. X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050. J. Vis. Exp. (159), e61288, doi:10.3791/61288 (2020).

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