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阿西尔-PEGyl交换凝胶移位测定，用于脑膜蛋白棕榈酸化定量测定
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Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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阿西尔-PEGyl交换凝胶移位测定，用于脑膜蛋白棕榈酸化定量测定

6,242 Views

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08:28 min

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March 29, 2020

DOI:

10.3791/61018-v

DOI:
10.3791/61018-v

•

08:28 min
March 29, 2020

•

9 Views
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1Department of Pharmacology,UC Davis School of Medicine

Summary

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棕榈化需要以可逆的方式将16碳棕榈酰化物与靶蛋白的半胱氨酸残留物结合。在这里，我们描述了一种生化方法，即acyl-PEGyl交换凝胶移位（APEGS）测定，以研究小鼠脑水化剂中任何感兴趣的蛋白质的棕榈酸化状态。

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Cite this Article

Speca, D. J., Diaz, E. Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins. J. Vis. Exp. (157), e61018, doi:10.3791/61018 (2020).

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