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Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

6,200 Views

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08:28 min

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March 29, 2020

DOI:

10.3791/61018-v

DOI:
10.3791/61018-v

•

08:28 min
March 29, 2020

•

2 Views
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1Department of Pharmacology,UC Davis School of Medicine

Tags

PalmitoylationBrain Membrane ProteinsAcyl-PEGyl ExchangeGel Shift AssayQuantitative DeterminationBiochemical MethodAffinity PurificationGel MobilityHomogenizationCentrifugationABCA AssayProtein QuantitationTCEPNEMChloroform-methanol Precipitation

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Speca, D. J., Diaz, E. Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins. J. Vis. Exp. (157), e61018, doi:10.3791/61018 (2020).

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