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Inmunología y contagio
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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

DOI:
10.3791/60806-v

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07:50 min

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March 30, 2020

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, , ,
1Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine,Northwestern University, 2Department of Pathology,University of Illinois at Chicago, 3Department of Microbiology and Immunology, Feinberg School of Medicine,Northwestern University

Capítulos

  • 00:05Introduction
  • 00:39Antibody and Fluorophore Selecting and Staining
  • 01:52Spectral Library Preparation
  • 02:59Multispectral Imaging
  • 03:54Spectral Unmixing
  • 04:37Analyzing Multispectral Images via Cell Segmentation and Phenotyping
  • 05:52Results: Representative Multispectral Imaging and Cell Quantification
  • 07:07Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a rapid staining method to perform multispectral imaging on frozen tissues.

Tags

Multispectral Fluorescence ImagingBiomarker DetectionCo-localizationRapid Staining MethodSpectral LibraryMachine LearningImage Analysis

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