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간 종양 세포의 맥락에서 자연 킬러 세포 매개 세포 독성 및 이동의 측정
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Medicina
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JoVE Revista Medicina
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
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간 종양 세포의 맥락에서 자연 킬러 세포 매개 세포 독성 및 이동의 측정

17,506 Views

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06:55 min

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February 22, 2020

DOI:

10.3791/60714-v

DOI:
10.3791/60714-v

•

06:55 min
February 22, 2020

•

6 Views
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1Department of Biochemistry and Molecular Genetics,University of Alabama at Birmingham

Tags

NK Cell FunctionNK Cell-mediated CytotoxicityNK Cell MigrationTumor MicroenvironmentLDH AssayCalcein AM AssayHuman Liver Cancer CellsCell CultureCentrifugation96-well PlateAbsorbance MeasurementCytotoxicity Calculation

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Chava, S., Bugide, S., Gupta, R., Wajapeyee, N. Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells. J. Vis. Exp. (156), e60714, doi:10.3791/60714 (2020).

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