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Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
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Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
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06:13 min
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February 21, 2020
DOI:
1Institute of Analytical Chemistry, Chemo- and Biosensors,University of Regensburg, 2Institute of Pharmacy,University of Regensburg, 3Department of Chemistry and Pharmacy,Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT
Summary
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This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.
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Stolwijk, J. A., Mildner, A., Kade, C., Skiba, M., Bernhardt, G., Buschauer, A., Huebner, H., Gmeiner, P., Wegener, J. Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays. J. Vis. Exp. (156), e60686, doi:10.3791/60686 (2020).
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