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Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

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06:13 min

February 21, 2020

DOI:

06:13 min
February 21, 2020

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Summary

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This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.

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