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Investigating Intestinal Barrier Breakdown in Living Organoids
 

Investigating Intestinal Barrier Breakdown in Living Organoids

Article DOI: 10.3791/60546-v • 07:18 min • March 26th, 2020
March 26th, 2020 •
1, 2, 1, 1, 2, 3, *1, *1,3
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg
* These authors contributed equally

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Resumen
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Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.

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Intestinal Barrier Breakdown Living Organoids Small Intestinal Organoids Assay Barrier Integrity Tight Junction Proteins Epithelial Cells Cell Culture Organoid Culture Centrifugation Tubes Cell Matrix Solution
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