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توصيف وظيفي لحلقه من نوع E3 اوبيكويتين الاربطه في المختبر وفي بلانتa
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Bioquímica
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JoVE Revista Bioquímica
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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توصيف وظيفي لحلقه من نوع E3 اوبيكويتين الاربطه في المختبر وفي بلانتa

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10:27 min

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December 05, 2019

DOI:

10.3791/60533-v

DOI:
10.3791/60533-v

•

10:27 min
December 05, 2019

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, , , , , ,
1Department of Entomology, Plant Pathology and Nematology,University of Idaho, 2Department of Plant Sciences,University of Idaho

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RING-type E3 Ubiquitin LigasesSite-directed MutagenesisIn Vitro Enzymatic ActivityIn Planta FunctionalityUbiquitination AssayRING DomainCys And His ResiduesPCRDpn1 DigestionPMAL-c2 VectorMBP TagSDS-PAGEPVDF MembraneWestern Blotting

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Kud, J., Wang, W., Yuan, Y., Caplan, A., Kuhl, J. C., Dandurand, L., Xiao, F. Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta. J. Vis. Exp. (154), e60533, doi:10.3791/60533 (2019).

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