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Whole-Mount In Situ Hybridisatie in Zebrafish Embryo's en Tube Formation Assay in iPSC-ECs om de rol van Endoglin in vasculaire ontwikkeling te bestuderen
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Whole-Mount In Situ Hybridisatie in Zebrafish Embryo's en Tube Formation Assay in iPSC-ECs om de rol van Endoglin in vasculaire ontwikkeling te bestuderen
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May 28, 2020
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1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University
Summary
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Hier gepresenteerd is een protocol voor whole-mount in situ RNA hybridisatie analyse in zebravis en buisvorming test in patiënt-afgeleide geïnduceerde pluripotente stamcel-afgeleide endotheelcellen om de rol van endoglin in vasculaire vorming te bestuderen.
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Wang, Y., Zhang, D., Zhou, F., Zhou, M., Li, Q., Chen, J., Yang, J. Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development. J. Vis. Exp. (159), e60498, doi:10.3791/60498 (2020).
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