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斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用
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May 28, 2020
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1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University
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本文介绍的是一种在斑马鱼和管状细胞中对斑马鱼进行全位RNA杂交分析的协议，在患者诱导的多能干细胞衍生内皮细胞中进行管状分析，以研究内索林在血管形成中的作用。
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Wang, Y., Zhang, D., Zhou, F., Zhou, M., Li, Q., Chen, J., Yang, J. Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development. J. Vis. Exp. (159), e60498, doi:10.3791/60498 (2020).
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