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伪多蒙多尼亚小殖民地文化及其藻酸盐的定量
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
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伪多蒙多尼亚小殖民地文化及其藻酸盐的定量

8,157 Views

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09:06 min

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February 22, 2020

DOI:

10.3791/60466-v

DOI:
10.3791/60466-v

•

09:06 min
February 22, 2020

•

5 Views
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1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Summary

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在这里，我们描述了一个生长条件，以培养伪多蒙多尼亚小殖民地变种。我们还描述了使用传统的尿酸卡巴佐尔测定法和基于藻酸盐的单克隆抗体（mAb）的ELISA检测和定量由P.aeruginosa产生的外糖藻酸盐的两种独立方法。

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Pseudomonas AeruginosaSmall Colony VariantSCVAlginateELISAUronic Acid CarbazolePhysiological ActivationSalvage PathwayOD600

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Cite this Article

Al Ahmar, R., Kirby, B. D., Yu, H. D. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (156), e60466, doi:10.3791/60466 (2020).

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