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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
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October 03, 2019
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Here we present a method to select for novel variants of the E. coli biotin-protein ligase BirA that biotinylates a specific target peptide. The protocol describes the construction of a plasmid for the bacterial display of the target peptide, generation of a BirA library, selection and characterization of BirA variants.
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Granhøj, J., Dimke, H., Svenningsen, P. Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes. J. Vis. Exp. (152), e60266, doi:10.3791/60266 (2019).
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