Journal
/
Bioingeniería
/
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
JoVE Revista
Bioingeniería
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioingeniería
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

DOI:
10.3791/60202-v

•

07:05 min

•

September 27, 2019

•

, , ,
1Institute of Biomedical Engineering and Nanomedicine,National Health Research Institutes, 2Ph.D. Program in Tissue Engineering and Regenerative Medicine,National Chung Hsing University, 3National Institute of Cancer Research,National Health Research Institutes

Capítulos

  • 00:04Título
  • 00:35PDMS Device Preparation for Multiple Single Cell Capture
  • 02:23Preparation of a Single-cell Suspension
  • 03:39Multiple Single-cell Capture & Triple Single-cell Capture
  • 04:48Results: Single Cell Capture and Cell Co-culture in the Hydrodynamic Shuttling Chip
  • 06:08Conclusion

Summary

Traducción Automática

Traducción Automática

This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.

Tags

Keywords: Single-cell Co-cultureMicrofluidic ChipPDMS Device FabricationCell CaptureCell-cell InteractionLive Cell ImagingDeterministic Cell Patterning

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/60202?article_type=v

A Microfluidic-based Hydrodynamic Trap for Single Particles
check_url/es/60202?article_type=v

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
check_url/es/60202?article_type=v

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
check_url/es/60202?article_type=v

A Microfluidic Chip for ICPMS Sample Introduction
check_url/es/60202?article_type=v

The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture
check_url/es/60202?article_type=v

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
check_url/es/60202?article_type=v

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
check_url/es/60202?article_type=v

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
check_url/es/60202?article_type=v

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
check_url/es/60202?article_type=v

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Read Article