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Journal / Developmental Biology / Dissektion, kultur og analyse af primær kranie…
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Developmental Biology

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JoVE Journal Developmental Biology
Dissektion, kultur og analyse af primær kranie neurale crest celler fra mus til studiet af Neural Crest celle delaminering og migration
 
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Dissektion, kultur og analyse af primær kranie neurale crest celler fra mus til studiet af Neural Crest celle delaminering og migration

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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Denne protokol beskriver dissektion og kultur af kranielle neurale crest celler fra musemodeller, primært til studiet af celle migration. Vi beskriver de anvendte Live imaging teknikker og analyse af hastighed og celle formændringer.

Tags

Udviklingsmæssige biologi cellekultur levende billeddannelse mus kranie neurale crest celle migration epitelial-mesenchymal overgang celle motilitet
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