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使用蝇S2细胞进行细胞分裂的活成像
JoVE Revista
Genética
This content is Free Access.
JoVE Revista Genética
Use of Drosophila S2 Cells for Live Imaging of Cell Division
DOI:

00:06 min

August 23, 2019

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Capítulos

  • 00:04Título
  • 00:26Fluorescent Protein Expression Induction and Cell Imaging Preparation
  • 01:24Live Cell Imaging Program Setup
  • 02:34Image Dividing Cells
  • 04:31Results: Representative Live Imaging of Cell Cycle Defects Due to Mitotic Checkpoint Activation in S2 Cells
  • 05:50Conclusion

Summary

Traducción Automática

使用荧光标记的蛋白质和延时显微镜可以实时可视化细胞分裂。使用此处介绍的协议,用户可以分析细胞分裂时序动力学、线粒主轴组件以及染色体的组装和分离。RNA干扰(RNAi)介导的基因敲除后这些事件的缺陷可以进行评估和量化。

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