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NMR-based activiteit assays voor het bepalen van samengestelde remming, IC50 waarden, artefactuele activiteit, en de hele cel activiteit van nucleoside Ribohydrolases
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June 30, 2019
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NMR-based Activity AssaysEnzyme InhibitionIC50 ValuesArtifact DetectionWhole-cell ActivityNucleoside RibohydrolasesFragment ScreeningTrichomonas VaginalisAnti-trichomonal DrugsReagent VolumesReaction ComponentsSubstrate PreparationTest Compound PreparationReaction Stock SolutionReaction InitiationReaction Quenching
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Stockman, B. J., Kaur, A., Persaud, J. K., Mahmood, M., Thuilot, S. F., Emilcar, M. B., Canestrari, M., Gonzalez, J. A., Auletta, S., Sapojnikov, V., Caravan, W., Muellers, S. N. NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases. J. Vis. Exp. (148), e59928, doi:10.3791/59928 (2019).
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