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RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
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RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)

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09:26 min

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July 10, 2019

DOI:

10.3791/59913-v

DOI:
10.3791/59913-v

•

09:26 min
July 10, 2019

•

11 Views
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1Department of Molecular Genetics, Center for RNA Biology,The Ohio State University

Summary

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在这里,我们提出一个协议,以丰富内源RNA结合位点或"足迹"的RNA:蛋白质(RNP)复合物从哺乳动物细胞。这种方法涉及RNP亚单位的两个免疫沉淀,因此被称为RNA免疫沉淀串联(RIPiT)。

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Woodward, L., Gangras, P., Singh, G. Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq). J. Vis. Exp. (149), e59913, doi:10.3791/59913 (2019).

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