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毛里小肠内皮淋巴细胞的内质成像
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
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毛里小肠内皮淋巴细胞的内质成像

7,604 Views

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08:00 min

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June 24, 2019

DOI:

10.3791/59853-v

DOI:
10.3791/59853-v

•

08:00 min
June 24, 2019

•

5 Views
,
1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine,Rutgers New Jersey Medical School

Summary

Automatically generated

我们描述了一种通过倒转旋转盘共聚焦显微镜,利用鼠小肠的细胞内成像来可视化GFP标记的IELs的方法。该技术能够跟踪粘液中的活细胞长达4小时,并可用于研究各种肠道免疫-皮皮相互作用。

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Cite this Article

Jia, L., Edelblum, K. L. Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine. J. Vis. Exp. (148), e59853, doi:10.3791/59853 (2019).

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