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Fremstilling af hele knoglemarv til masse cytometri analyse af neutrofile-Lineage celler
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JoVE Revista Inmunología y contagio
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Fremstilling af hele knoglemarv til masse cytometri analyse af neutrofile-Lineage celler

DOI:
10.3791/59617-v

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08:09 min

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June 19, 2019

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, , , , , , ,
1Division of Inflammation Biology,La Jolla Institute for Immunology, 2Flow Cytometry Core Facility,La Jolla Institute for Immunology

Capítulos

  • 00:04Título
  • 01:41Bone Marrow (BM) Harvest
  • 03:40BM Cell Staining and Mass Cytometry (CyTOF) Analysis Preparation
  • 06:15Results: Representative Whole Bone Marrow CyTOF Analysis of Neutrophil-lineage Cells
  • 07:47Conclusion

Summary

Traducción Automática

Traducción Automática

Her præsenterer vi en protokol til at behandle frisk knoglemarv (BM) isoleret fra mus eller human for high-dimensionelle masse cytometri (flowcytometri af Time-of-Flight, cytof) analyse af neutrofile-Lineage celler.

Tags

Mass CytometryBone MarrowNeutrophil-lineage CellsMyeloid CellsCell PreservationC57 Black Six MouseTibiaFemurDissection

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