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다각형 광 산란에 결합된 크기 배제 크로마토그래피에 의한 단백질의 특성 (SEC-MALS)
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Bioquímica
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JoVE Revista Bioquímica
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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다각형 광 산란에 결합된 크기 배제 크로마토그래피에 의한 단백질의 특성 (SEC-MALS)

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10:00 min

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June 20, 2019

DOI:

10.3791/59615-v

DOI:
10.3791/59615-v

•

10:00 min
June 20, 2019

•

71 Views
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1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Tags

SEC-MALSSize-exclusion ChromatographyMulti-angle Light ScatteringMolecular WeightMacromoleculesOligomersComplexesGlycoproteinsMembrane ProteinsAbsolute MethodPeptidesPolymersProteinsNucleocapsidsPolysaccharidesVirus-like ParticlesVirusesPhosphate-buffered SalineFlow RateDRI Flow CellPurge ModeSystem CleanlinessRefractive Index

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Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).

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