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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
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Medio ambiente
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JoVE Revista Medio ambiente
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

DOI:
10.3791/59562-v

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08:05 min

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October 07, 2020

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, , , , , ,
1Department of Biological Sciences, Faculty of Science and Engineering,Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences,Tokyo Metropolitan University, 3Center for Ecological Research,Kyoto University, 4Department of Chemistry, Faculty of Science,Toho University, 5Graduate School of Integrated Arts and Sciences,Hiroshima University

Capítulos

  • 00:04Introduction
  • 01:36Preparation of a PTFE Envelope for Quantitatively Capturing Gaseous NH3
  • 02:41Capturing Diffused NH4+ in 2M H2SO4 absorbed to the GF/D Filter in the PTFE Envelope and the Persulfate Oxidation
  • 04:53Determining the NO3” Converted from NH4+ by the Denitrifier Method Using Quadrupole GC/MS
  • 06:38Results: Determination of DNRA Rate Based on 14NH4+/15NH4+ Analyses
  • 07:36Conclusion

Summary

Traducción Automática

Traducción Automática

A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.

Tags

DNRANitrate ReductionAmmoniumN15 AnalysisN2OGas Chromatography-mass SpectrometryCoastal EcosystemsAquatic EnvironmentsDenitrificationAnammox

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