Journal
/
Quimica
/
Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941
JoVE Revista
Quimica
This content is Free Access.
JoVE Revista Quimica
Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

DOI:
10.3791/59557-v

•

09:50 min

•

April 28, 2019

•

, , , , , , ,
1Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine,Medical University of Vienna, 2Department for Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences,University of Vienna, 3Ludwig Boltzmann Institute Applied Diagnostics, 4CBmed GmbH – Center for Biomarker Research in Medicine

Capítulos

  • 00:04Título
  • 01:20Automated Synthesis of [11C]SNAP-7941 for Preclinical Use
  • 04:40Quality Control (QC) of [11C]SNAP-7941
  • 05:55Real-Time Kinetic Assay
  • 08:02Results: Evaluation of [11C]SNAP-7941 Interactions Towards the Permeability Glycoprotein 1 (P-gp) Transporter
  • 08:44Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we represent a protocol for the fully automated radiolabelling of [11C]SNAP-7941 and the analysis of the real-time kinetics of this PET-tracer on P-gp expressing and non-expressing cells.

Tags

[11C]SNAP-7941Carbon-11 RadiolabelingPETMelanin-concentrating Hormone ReceptorEfflux TransportersAutomated RadiosynthesisMethyl Group

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Protocol for the Synthesis of Ortho-trifluoromethoxylated Aniline Derivatives

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Read Article