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增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量

8,127 Views

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07:00 min

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June 04, 2019

DOI:

10.3791/59545-v

DOI:
10.3791/59545-v

•

07:00 min
June 04, 2019

•

13 Views
, , ,
1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Summary

Automatically generated

这里介绍的是一个协议,用于测量增殖CD4+ T细胞,以响应抗原蛋白或肽使用染料稀释。这种测定对稀有抗原特异性T细胞特别敏感,可以进行改性,以利于抗原特异性细胞的克隆。

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Cite this Article

Di Carluccio, A. R., Tresoldi, E., So, M., Mannering, S. I. Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester. J. Vis. Exp. (148), e59545, doi:10.3791/59545 (2019).

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