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原子吸纳光谱测量哺乳动物细胞细胞内锌池
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JoVE Revista Bioquímica
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
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原子吸纳光谱测量哺乳动物细胞细胞内锌池

38,122 Views

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13:04 min

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May 16, 2019

DOI:

10.3791/59519-v

DOI:
10.3791/59519-v

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13:04 min
May 16, 2019

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1 Views
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1Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Department of Chemistry,Skidmore College, 3Candiac MR Center,Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Tags

Atomic Absorbance SpectroscopyIntracellular ZincMammalian CellsTransition MetalsMetal HomeostasisGraphite Furnace Atomic Absorption Spectroscopy (GF-AAS)Trace Element AnalysisMetal MisbalancesPathological ConditionsMethod DevelopmentSample PreparationLow Sample VolumeMethod Validation

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Gordon, S. J., Xiao, Y., Paskavitz, A. L., Navarro-Tito, N., Navea, J. G., Padilla-Benavides, T. Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells. J. Vis. Exp. (147), e59519, doi:10.3791/59519 (2019).

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