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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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00:09 min
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August 21, 2019
DOI:
1Stream Biofilm and Ecosystem Research Laboratory, School of Architecture, Civil and Environmental Engineering,Ecole Polytechnique Fédérale de Lausanne, 2Plateforme technique, School of Architecture, Civil and Environmental Engineering,Ecole Polytechnique Fédérale de Lausanne, 3Water Chemistry and Water Technology, Engler-Bunte-Institut,Karlsruhe Institute of Technology, 4Thorlabs GmbH
Summary
Automatically generated
Microbial biofilms form complex architectures at interphases and develop into highly scale-dependent spatial patterns. Here, we introduce an experimental system (hard- and software) for the automated acquisition of 3D optical coherence tomography (OCT) datasets. This toolset allows the non-invasive and multi-scale characterization of biofilm morphogenesis in space and time.
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Cite this Article
Depetris, A., Wiedmer, A., Wagner, M., Schäfer, S., Battin, T. J., Peter, H. Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales. J. Vis. Exp. (150), e59356, doi:10.3791/59356 (2019).
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