Journal
/
Bioingeniería
/
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
JoVE Revista
Bioingeniería
This content is Free Access.
JoVE Revista Bioingeniería
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

DOI:
10.3791/59324-v

•

12:56 min

•

May 13, 2019

•

1Biology Department,Luminex Corporation

Capítulos

  • 00:04Título
  • 00:33Clastogen and/or Aneugen Exposure
  • 01:49Sample Processing
  • 03:50Multispectral Imaging Flow Cytometry (MIFC)
  • 05:15Binucleated Cell (BNC) Identification
  • 06:48Micronucleus (MN) Identification
  • 07:53Polynucleated Population Identification
  • 08:41BNC and MN Mask Examination
  • 09:52Statistics Table Creation
  • 10:31Experimental File Batch Processing
  • 11:10Results: Representative Cell Scoring and Cytotoxicity Quantification
  • 12:23Conclusion

Summary

Traducción Automática

Traducción Automática

The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay using manual microscopy is laborious and suffers from subjectivity and inter-scorer variability. This paper describes the protocol developed to perform a fully automated version of the assay using multispectral imaging flow cytometry.

Tags

In Vitro Micronucleus AssayMultispectral Imaging Flow CytometryAutomated MethodGenotoxicityCytotoxicityClastogensAneugensCell ExposureCytochalasin BPotassium ChlorideFormalinHoechst 33342High-throughputAutomated MicroscopyConventional Flow Cytometry

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Ovarian Cancer Detection Using Photoacoustic Flow Cytometry

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Read Article