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Elettroforesi ibrida trasparente/blu nativa per la separazione e l'analisi dei supercomplessi di catene respiratorie mitocondriali
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Bioquímica
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JoVE Revista Bioquímica
Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
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Elettroforesi ibrida trasparente/blu nativa per la separazione e l'analisi dei supercomplessi di catene respiratorie mitocondriali

12,603 Views

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11:25 min

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May 19, 2019

DOI:

10.3791/59294-v

DOI:
10.3791/59294-v

•

11:25 min
May 19, 2019

•

13 Views
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1Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine,University of Ottawa

Tags

Mitochondrial Respiratory Chain SupercomplexesBlue Native PAGEClear Native PAGEHybrid Clear/blue Native ElectrophoresisMitochondrial IsolationDigitonin ExtractionGradient Gel Electrophoresis

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Cuillerier, A., Burelle, Y. Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes. J. Vis. Exp. (147), e59294, doi:10.3791/59294 (2019).

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