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Cancer Research

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JoVE Journal Cancer Research
Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
 

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

Article DOI: 10.3791/59278-v • 09:17 min • May 3rd, 2019
May 3rd, 2019 •
1, 1
1Cancer Genomics Netherlands, Prinses Máxima Center for Pediatric Oncology

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Here we describe a method for high-resolution time-lapse multiphoton imaging of brain tumor cells before and after invasive surgical intervention (e.g., biopsy) within the same living animal. This method allows studying the impact of these invasive surgical procedures on tumor cells' migratory, invasive, and proliferative behavior at a single cell level.

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