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哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究

6,291 Views

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10:05 min

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March 05, 2019

DOI:

10.3791/59215-v

DOI:
10.3791/59215-v

•

10:05 min
March 05, 2019

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5 Views
, ,
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST),Korea Advanced Institute of Science and Technology (KAIST)

Summary

Automatically generated

我们提出了利用 hela 细胞研究哺乳动物细胞周期中双链 rna 结合蛋白激酶 rna 激活 (pkr) 的 rna 相互作用的实验方法。该方法利用甲醛对交联 rna-pkr 复合物和免疫沉淀进行富集 pkr 约束 rna。这些 rna 可以通过高通量测序或 qrt-pcr 进一步分析。

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Cite this Article

Kim, S., Kang, M., Kim, Y. Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle. J. Vis. Exp. (145), e59215, doi:10.3791/59215 (2019).

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