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Xylem vatten distribution i Woody växter visualiseras med en Cryo-scanning Electron Mikroskop
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1Department of Plant Ecology,Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center,Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing,Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture,Kyushu University, 5Department of Bacteriology,University of Wisconsin, 6Research Faculty of Agriculture,Hokkaido University
Capítulos
- 00:04Título
- 01:14Sampling: Tension Relaxation within Water Column of Xylem Conduits
- 03:00Freeze Fixation with Liquid Nitrogen
- 04:04Specimen Preparation
- 05:49Transfer to the Cryo-SEM Specimen Chamber
- 06:30Freeze-etching
- 07:00Results: Xylem Water Distribution in Woody Plants
- 09:04Conclusion
Observation av vatten distributionen inom Xylem ger viktig information om vattenflödesdynamik i vedartade växter. I denna studie visar vi den praktiska metoden för att observera Xylem vatten distribution in situ genom att använda en kryostat och Cryo-SEM, vilket eliminerar artifaktuella förändringar i vatten status under prov beredning.
Tags
XylemWater DistributionCryo-scanning Electron MicroscopeFreeze-fixationXylem TensionWater PotentialCavitationXylem CellsAerotomeWoody PlantsWater RegimeSeasonal VariationBiotic StressFreeze/thaw CycleWet WoodSampling ProcedurePressure ChamberRelaxation TreatmentWater SurfaceTranspirationVessel Length
