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デジタルPCRを用いたマウス組織における異種マウスモデルにおける移植片対宿主病の誘導とスコア付け
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Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
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デジタルPCRを用いたマウス組織における異種マウスモデルにおける移植片対宿主病の誘導とスコア付け

6,746 Views

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06:06 min

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May 23, 2019

DOI:

10.3791/59107-v

DOI:
10.3791/59107-v

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06:06 min
May 23, 2019

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6 Views
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1Department of Microbiology, Molecular Genetics, and Immunology,University of Kansas Medical Center

Tags

Xenograft-versus-host DiseaseXenoGVHDNSG MiceIrradiationPeripheral Blood Mononuclear CellsPBMCsDensity Gradient CentrifugationRetro-orbital InjectionHuman T CellsImmunosuppressive Therapies

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Cite this Article

Seng, A., Markiewicz, M. A. Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR. J. Vis. Exp. (147), e59107, doi:10.3791/59107 (2019).

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