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Induktion og scoring af graft-versus-Host-sygdom i en xenogen Murinmodel og kvantificering af humane T-celler i muse væv ved hjælp af digital PCR
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JoVE Revista Inmunología y contagio
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Induktion og scoring af graft-versus-Host-sygdom i en xenogen Murinmodel og kvantificering af humane T-celler i muse væv ved hjælp af digital PCR

6,746 Views

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06:06 min

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May 23, 2019

DOI:

10.3791/59107-v

DOI:
10.3791/59107-v

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06:06 min
May 23, 2019

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6 Views
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1Department of Microbiology, Molecular Genetics, and Immunology,University of Kansas Medical Center

Tags

Xenograft-versus-host DiseaseXenoGVHDNSG MiceIrradiationPeripheral Blood Mononuclear CellsPBMCsDensity Gradient CentrifugationRetro-orbital InjectionHuman T CellsImmunosuppressive Therapies

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Seng, A., Markiewicz, M. A. Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR. J. Vis. Exp. (147), e59107, doi:10.3791/59107 (2019).

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