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1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York
Capítulos
- 00:04Título
- 01:00Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
- 03:02Cell Lysis and Protein Transfer
- 05:28Histone Post-translational Modification Detection
- 06:52Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
- 08:03Conclusion
Denne protokol beskriver eksperimentelle procedurer for at karakterisere genome-wide ændringer i niveauet af Histon posttranslationelle modifikationer (PTM) forekommer i forbindelse med overekspression af proteiner er forbundet med ALS og Parkinsons sygdom i Saccharomyces cerevisiae modeller. Efter SDS-PAGE adskillelse registreres individuelle Histon PTM niveauer med modifikation-specifikke antistoffer via Western blotting.
