Journal
/
Quimica
/
合成と化反応のサイト選択的に作成するためのチオール反応性の試薬の変更 Immunoconjugates
JoVE Revista
Quimica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Quimica
Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
Please note that all translations are automatically generated. Click here for the English version.

合成と化反応のサイト選択的に作成するためのチオール反応性の試薬の変更 Immunoconjugates

9,076 Views

•

08:47 min

•

March 06, 2019

DOI:

10.3791/59063-v

DOI:
10.3791/59063-v

•

08:47 min
March 06, 2019

•

2 Views
, , ,
1Department of Chemistry,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Cancer Center, 4Department of Radiology,Weill Cornell Medical College

Summary

Automatically generated

このプロトコルではポッド、phenyoxadiazolyl メチル スルホンに基づく生体分子、特に抗体のチオールに貨物のサイト選択的添付試薬の合成について述べる。また、合成とポッド ベアリング二官能性キレート剤とその活用モデル抗体の特性を説明します。

Explore More Videos

Thiol-reactive ReagentsSite-specific BioconjugationImmunoconjugatesPODSMaleimide-thiol-based BioconjugationAminophenyl Oxadiazole ThiolDiisopropylethylamineIodomethaneBoc-protected Carboxylic AcidDichloromethaneEDCIAnilineHydrochloric AcidSodium CarbonateMagnesium SulfateEthyl AcetateCyclohexaneCarbamateM-chloroperbenzoic Acid

Read Article

Cite this Article

Davydova, M., Dewaele Le Roi, G., Adumeau, P., Zeglis, B. M. Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates. J. Vis. Exp. (145), e59063, doi:10.3791/59063 (2019).

Download .ris file

Copy

Share Video

.

Copy