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Celle fraktionering af U937 celler i mangel af højhastighedstog centrifugering

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Celle fraktionering af U937 celler i mangel af højhastighedstog centrifugering

12,919 Views

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06:53 min

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January 18, 2019

DOI:

10.3791/59022-v

DOI:

10.3791/59022-v

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06:53 min

January 18, 2019

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13 Views

William D. McCaig, Matthew A. Deragon, Robert J. Haluska Jr,, Alexa L. Hodges, Payal S. Patel, Timothy J. LaRocca

¹Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Tags

Cell Fractionation U937 Cells High-speed Centrifugation Protocol Subcellular Fractions Cytoplasm Mitochondria Plasma Membrane Hypotonic Buffers Digitonin Mechanical Lysis Differential Centrifugation Protein Localization Inexpensive Straightforward

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