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サイト指示された突然変異誘発のため生体外でそしてエシェリヒア属大腸菌の RNA 相互作用に代表される生体実験
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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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サイト指示された突然変異誘発のため生体外でそしてエシェリヒア属大腸菌の RNA 相互作用に代表される生体実験

19,159 Views

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07:04 min

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February 05, 2019

DOI:

10.3791/58996-v

DOI:
10.3791/58996-v

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07:04 min
February 05, 2019

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7 Views
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1Department of Biochemistry and Molecular Biology,University of Southern Denmark

Tags

Site-directed MutagenesisRNA InteractionsEscherichia ColiPCRDNA TemplatePrimer PairsAgarose Gel ElectrophoresisDNA PurificationSpectrophotometry2-step PCR3-step PCRRNA-RNA InteractionsRNA-protein Interactions

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Andreassen, P. R., Pettersen, J. S., Jørgensen, M. Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli. J. Vis. Exp. (144), e58996, doi:10.3791/58996 (2019).

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