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Un dosage de cytotoxicité à base de plaques pour l’évaluation de la fonction cytolytique des cellules tueuses naturelles du rat placentaire

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

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Un dosage de cytotoxicité à base de plaques pour l’évaluation de la fonction cytolytique des cellules tueuses naturelles du rat placentaire

5,643 Views

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10:44 min

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June 02, 2019

DOI:

10.3791/58961-v

DOI:

10.3791/58961-v

•

10:44 min

June 02, 2019

•

2 Views

Cedar H. Baik, Mallory Geer, Olivia K. Travis, Denise C. Cornelius²

¹Department of Emergency Medicine,University of Mississippi Medical Center, ²Department of Experimental Therapeutics and Pharmacology,University of Mississippi Medical Center

Tags

– Plate-based Cytotoxicity Assay- Rat Placental Natural Killer Cells- Cytolytic Function- Cytotoxic Activity- Inexpensive Method- Specialized Equipment- Hazardous Materials- CD8+ Cytolytic Cells- Target-effector Cell Ratio Optimization- Mononuclear Cell Isolation- Placenta Homogenization- Density Gradient Centrifugation- Biotin-labeled CD3 Antibody- Magnetic Bead Separation

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