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电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定
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JoVE Revista Bioquímica
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定

7,288 Views

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12:15 min

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February 21, 2019

DOI:

10.3791/58896-v

DOI:
10.3791/58896-v

•

12:15 min
February 21, 2019

•

11 Views
, ,
1School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology,University of Leeds, 2Department of Chemistry and Nano-Science Center,University of Copenhagen

Summary

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在这里, 我们提出了一个方案, 研究质子在单个脂质体脂质膜上的分子转移机制, 以细胞色素b3 为例。结合电化学和荧光显微镜, 可以单独检测和分析含有单一或多种酶的单囊腔内的 ph 值变化。

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Cite this Article

Mazurenko, I., Hatzakis, N. S., Jeuken, L. J. Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy. J. Vis. Exp. (144), e58896, doi:10.3791/58896 (2019).

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