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JoVE Journal Neuroscience
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
 

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

Article DOI: 10.3791/58870-v • 06:32 min • March 23rd, 2019
March 23rd, 2019 •
1,2, 1, 3, 1
1Center for Perinatal Biology, Department of Basic Sciences, Loma Linda University, 2Department of Biology, The College of New Jersey, 3Department of Neurosurgery, Loma Linda University

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This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.

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Rhythmically-active In Vitro Neonatal Rodent Brainstem Spinal Cord Thin Slice Dissection Slicing Mirian Medullary Respiratory Network Experimental Flexibility Electrophysiology Experiments Neural Control Circuit Breathing Rhythms Pathology Disregulation Of Breathing Genetically Modified Rodents Aerated Dissection Chamber Dissection Microscope Tissue Dorsal-side Up Rostral End Midsagittal Incision Parietal Suture Occipital Sutures Bone Flaps Cerebral Cortex Excision Caudal Portion Of Cerebellum Dorsal Laminectomy
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