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重组融合蛋白在荧光蛋白酶检测平台中的应用及其凝胶内再生
JoVE Revista
Bioquímica
Author Produced
This content is Free Access.
JoVE Revista Bioquímica
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
DOI:

19:23 min

January 16, 2019

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Capítulos

  • 00:00Título
  • 01:05Generation of the Substrate-coding Expression Plasmids
  • 02:16Expression of the Fluorescent Substrates
  • 03:35Cell Disruption
  • 04:26Ni-NTA Magnetic Agarose Bead-based Protease Assay
  • 12:39PAGE Analysis
  • 14:41Representative Results
  • 18:29Conclusion

Summary

Traducción Automática

在这里, 我们提出了一个最近开发的蛋白酶分析平台的详细程序, 利用 n-端六利三烷基-马氏体结合蛋白和荧光蛋白融合重组基板附着在镍硝基三乙酸表面酸性磁性琼脂糖珠。并对十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的测定样品进行了凝胶分析。

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