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An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
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Medio ambiente
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JoVE Revista Medio ambiente
An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

15,314 Views

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07:45 min

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October 22, 2018

DOI:

10.3791/58674-v

DOI:
10.3791/58674-v

•

07:45 min
October 22, 2018

•

27 Views
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1Department of Plant Sciences,University of California at Davis, 2Department of Natural Resources and the Environment,University of New Hampshire

Summary

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Visualizing and measuring root growth in situ is extremely challenging. We present a customizable rhizobox method to track root development and proliferation over time in response to nutrient enrichment. This method is used to analyze maize genotypic differences in root plasticity in response to an organic nitrogen source.

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Schmidt, J. E., Lowry, C., Gaudin, A. C. An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients. J. Vis. Exp. (140), e58674, doi:10.3791/58674 (2018).

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